High Throughput Screening: Publications

  • Postigo, A; Ramsden, AE; Howell, M and Way, M (2017)
    Cytoplasmic ATR activation promotes vaccinia virus genome replication.
    Cell Reports 19, 1022-1032 PubMed abstract
  • Sansregret, L; Patterson, JO; Dewhurst, S; López-García, C; Koch, A; McGranahan, N; Chao, WCH; Barry, DJ; Rowan, A; Instrell, R; Horswell, S; Way, M; Howell, M; Singleton, MR; Medema, RH; Nurse, P; Petronczki, M and Swanton, C (2017)
    APC/C dysfunction limits excessive cancer chromosomal instability.
    Cancer Discovery 7, 218-233 PubMed abstract
  • Takaki, T; Montagner, M; Serres, MP; Le Berre, M; Russell, M; Collinson, L; Szuhai, K; Howell, M; Boulton, SJ; Sahai, E and Petronczki, M (2017)
    Actomyosin drives cancer cell nuclear dysmorphia and threatens genome stability.
    Nature Communications 8, 16013 PubMed abstract
  • Williamson, L; Saponaro, M; Boeing, S; East, P; Mitter, R; Kantidakis, T; Kelly, GP; Lobley, A; Walker, J; Spencer-Dene, B; Howell, M; Stewart, A and Svejstrup, JQ (2017)
    UV irradiation induces a non-coding RNA that functionally opposes the protein encoded by the same gene.
    Cell 168, 843-855 PubMed abstract
  • Boeing, S; Williamson, L; Encheva, V; Gori, I; Saunders, RE; Instrell, R; Aygün, O; Rodriguez-Martinez, M; Weems, JC; Kelly, GP; Conaway, JW; Conaway, RC; Stewart, A; Howell, M; Snijders, AP and Svejstrup, JQ (2016)
    Multiomic analysis of the UV-induced DNA damage response.
    Cell Reports 15, 1597-1610 PubMed abstract
  • Quétier, I; Marshall, JJT; Spencer-Dene, B; Lachmann, S; Casamassima, A; Franco, C; Escuin, S; Worrall, JT; Baskaran, P; Rajeeve, V; Howell, M; Copp, AJ; Stamp, G; Rosewell, I; Cutillas, P; Gerhardt, H; Parker, PJ and Cameron, AJM (2016)
    Knockout of the PKN family of Rho effector kinases reveals a non-redundant role for PKN2 in developmental mesoderm expansion.
    Cell Reports 14, 440-448 PubMed abstract
  • Baenke, F; Dubuis, S; Brault, C; Weigelt, B; Dankworth, B; Griffiths, B; Jiang, M; Mackay, A; Saunders, B; Spencer-Dene, B; Ros, S; Stamp, G; Reis-Filho, JS; Howell, M; Zamboni, N and Schulze, A (2015)
    Functional screening identifies MCT4 as a key regulator of breast cancer cell metabolism and survival.
    Journal of Pathology 237, 152-165 PubMed abstract
  • Dombernowsky, SL; Samsøe-Petersen, J; Petersen, CH; Instrell, R; Hedegaard, A-MB; Thomas, L; Atkins, KM; Auclair, S; Albrechtsen, R; Mygind, KJ; Fröhlich, C; Howell, M; Parker, P; Thomas, G and Kveiborg, M (2015)
    The sorting protein PACS-2 promotes ErbB signalling by regulating recycling of the metalloproteinase ADAM17.
    Nature Communications 6, 7518 PubMed abstract
  • Joachim, J; Jiang, M; McKnight, NC; Howell, M and Tooze, SA (2015)
    High-throughput screening approaches to identify regulators of mammalian autophagy.
    Methods 75, 96-104 PubMed abstract
  • Schug, ZT; Peck, B; Jones, DT; Zhang, Q; Grosskurth, S; Alam, IS; Goodwin, LM; Smethurst, E; Mason, S; Blyth, K; McGarry, L; James, D; Shanks, E; Kalna, G; Saunders, RE; Jiang, M; Howell, M; Lassailly, F; Thin, MZ; Spencer-Dene, B; Stamp, G; van den Broek, NJF; Mackay, G; Bulusu, V; Kamphorst, JJ; Tardito, S; Strachan, D; Harris, AL; Aboagye, EO; Critchlow, SE; Wakelam, MJO; Schulze, A and Gottlieb, E (2015)
    Acetyl-CoA synthetase 2 promotes acetate utilization and maintains cancer cell growth under metabolic stress.
    Cancer Cell 27, 57-71 PubMed abstract
  • de Bruin, EC; Cowell, C; Warne, PH; Jiang, M; Saunders, RE; Melnick, MA; Gettinger, S; Walther, Z; Wurtz, A; Heynen, GJ; Heideman, DAM; Gómez-Román, J; García-Castaño, A; Gong, Y; Ladanyi, M; Varmus, H; Bernards, R; Smit, EF; Politi, K and Downward, J (2014)
    Reduced NF1 expression confers resistance to EGFR inhibition in lung cancer.
    Cancer Discovery 4, 606-619 PubMed abstract
  • Endesfelder, D; Burrell, RA; Kanu, N; McGranahan, N; Howell, M; Parker, PJ; Downward, J; Swanton, C and Kschischo, M (2014)
    Chromosomal instability selects gene copy-number variants encoding core regulators of proliferation in ER+ breast cancer.
    Cancer Research 74, 4853-4863 PubMed abstract
  • Staples, CJ; Myers, KN; Beveridge, RDD; Patil, AA; Howard, AE; Barone, G; Lee, AJX; Swanton, C; Howell, M; Maslen, S; Skehel, JM; Boulton, SJ and Collis, SJ (2014)
    Ccdc13 is a novel human centriolar satellite protein required for ciliogenesis and genome stability.
    Journal of Cell Science 127, 2910-2919 PubMed abstract
  • Sundaramoorthy, S; Vázquez-Novelle, MD; Lekomtsev, S; Howell, M and Petronczki, M (2014)
    Functional genomics identifies a requirement of pre-mRNA splicing factors for sister chromatid cohesion.
    EMBO Journal 33, 2623-2642 PubMed abstract
  • Burrell, RA; McClelland, SE; Endesfelder, D; Groth, P; Weller, M-C; Shaikh, N; Domingo, E; Kanu, N; Dewhurst, SM; Gronroos, E; Chew, SK; Rowan, AJ; Schenk, A; Sheffer, M; Howell, M; Kschischo, M; Behrens, A; Helleday, T; Bartek, J; Tomlinson, IP and Swanton, C (2013)
    Replication stress links structural and numerical cancer chromosomal instability.
    Nature 494, 492-496 PubMed abstract
  • Saunders, RE; Instrell, R; Rispoli, R; Jiang, M and Howell, M (2013)
    HTS-DB: an online resource to publish and query data from functional genomics high-throughput siRNA screening projects.
    Database 2013, bat072 PubMed abstract
  • Siebring-van Olst, E; Vermeulen, C; de Menezes, RX; Howell, M; Smit, EF and van Beusechem, VW (2013)
    Affordable luciferase reporter assay for cell-based high-throughput screening.
    Journal of Biomolecular Screening 18, 453-461 PubMed abstract
  • Wehr, MC; Holder, MV; Gailite, I; Saunders, RE; Maile, TM; Ciirdaeva, E; Instrell, R; Jiang, M; Howell, M; Rossner, MJ and Tapon, N (2013)
    Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila.
    Nature Cell Biology 15, 61-71 PubMed abstract
  • Gerlinger, M; Santos, CR; Spencer-Dene, B; Martinez, P; Endesfelder, D; Burrell, RA; Vetter, M; Jiang, M; Saunders, RE; Kelly, G; Dykema, K; Rioux-Leclercq, N; Stamp, G; Patard, JJ; Larkin, J; Howell, M and Swanton, C (2012)
    Genome-wide RNA interference analysis of renal carcinoma survival regulators identifies MCT4 as a Warburg effect metabolic target.
    Journal of Pathology 227, 146-156 PubMed abstract
  • Lee, AJX; Roylance, R; Sander, J; Gorman, P; Endesfelder, D; Kschischo, M; Jones, NP; East, P; Nicke, B; Spassieva, S; Obeid, LM; Birkbak, NJ; Szallasi, Z; McKnight, NC; Rowan, AJ; Speirs, V; Hanby, AM; Downward, J; Tooze, SA and Swanton, C (2012)
    CERT depletion predicts chemotherapy benefit and mediates cytotoxic and polyploid-specific cancer cell death through autophagy induction.
    Journal of Pathology 226, 482-494 PubMed abstract
  • McKnight, NC; Jefferies, HBJ; Alemu, EA; Saunders, RE; Howell, M; Johansen, T and Tooze, SA (2012)
    Genome-wide siRNA screen reveals amino acid starvation-induced autophagy requires SCOC and WAC.
    EMBO Journal 31, 1931-1946 PubMed abstract
  • Milanovic, M; Radtke, S; Peel, N; Howell, M; Carrière, V; Joffre, C; Kermorgant, S and Parker, PJ (2012)
    Anomalous inhibition of c-Met by the kinesin inhibitor aurintricarboxylic acid.
    International Journal of Cancer 130, 1060-1070 PubMed abstract
  • Ros, S; Santos, CR; Moco, S; Baenke, F; Kelly, G; Howell, M; Zamboni, N and Schulze, A (2012)
    Functional metabolic screen identifies 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 as an important regulator of prostate cancer cell survival.
    Cancer Discovery 2, 328-343 PubMed abstract
  • Rosse, C; Boeckeler, K; Linch, M; Radtke, S; Frith, D; Barnouin, K; Morsi, AS; Hafezparast, M; Howell, M and Parker, PJ (2012)
    Binding of dynein intermediate chain 2 to paxillin controls focal adhesion dynamics and migration.
    Journal of Cell Science 125, 3733-3738 PubMed abstract
  • Staples, CJ; Myers, KN; Beveridge, RDD; Patil, AA; Lee, AJX; Swanton, C; Howell, M; Boulton, SJ and Collis, SJ (2012)
    The centriolar satellite protein Cep131 is important for genome stability.
    Journal of Cell Science 125, 4770-4779 PubMed abstract
  • Steckel, M; Molina-Arcas, M; Weigelt, B; Marani, M; Warne, PH; Kuznetsov, H; Kelly, G; Saunders, B; Howell, M; Downward, J and Hancock, DC (2012)
    Determination of synthetic lethal interactions in KRAS oncogene-dependent cancer cells reveals novel therapeutic targeting strategies.
    Cell Research 22, 1227-1245 PubMed abstract
  • Jiang, M; Instrell, R; Saunders, B; Berven, H and Howell, M (2011)
    Tales from an academic RNAi screening facility; FAQs.
    Briefings in Functional Genomics 10, 227-237 PubMed abstract
  • Kveiborg, M; Instrell, R; Rowlands, C; Howell, M and Parker, PJ (2011)
    PKCα and PKCδ regulate ADAM17-mediated ectodomain shedding of heparin binding-EGF through separate pathways.
    PLOS ONE 6, e17168 PubMed abstract
  • Lee, AJX; Endesfelder, D; Rowan, AJ; Walther, A; Birkbak, NJ; Futreal, PA; Downward, J; Szallasi, Z; Tomlinson, IPM; Howell, M; Kschischo, M and Swanton, C (2011)
    Chromosomal instability confers intrinsic multidrug resistance.
    Cancer Research 71, 1858-1870 PubMed abstract
  • Wu, MY; Ramel, M-C; Howell, M and Hill, CS (2011)
    SNW1 is a critical regulator of spatial BMP activity, neural plate border formation, and neural crest specification in vertebrate embryos.
    PLOS Biology 9, e1000593 PubMed abstract
  • Boeckeler, K; Rosse, C; Howell, M and Parker, PJ (2010)
    Manipulating signal delivery - plasma-membrane ERK activation in aPKC-dependent migration.
    Journal of Cell Science 123, 2725-2732 PubMed abstract
  • Nishimura, AL; Župunski, V; Troakes, C; Kathe, C; Fratta, P; Howell, M; Gallo, J-M; Hortobágyi, T; Shaw, CE and Rogelj, B (2010)
    Nuclear import impairment causes cytoplasmic trans-activation response DNA-binding protein accumulation and is associated with frontotemporal lobar degeneration.
    Brain 133, 1763-1771 PubMed abstract
  • Swanton, C; Larkin, JM; Gerlinger, M; Eklund, AC; Howell, M; Stamp, G; Downward, J; Gore, M; Futreal, PA; Escudier, B; Andre, F; Albiges, L; Beuselinck, B; Oudard, S; Hoffmann, J; Gyorffy, B; Torrance, CJ; Boehme, KA; Volkmer, H; Toschi, L; Nicke, B; Beck, M and Szallasi, Z (2010)
    Predictive biomarker discovery through the parallel integration of clinical trial and functional genomics datasets.
    Genome Medicine 2, 53 PubMed abstract

[Page last updated 1 Nov 2017]

Michael Howell (Lead) 

michael.howell@crick.ac.uk
+44 (0) 20 7269 3170