Hasan Yardimci

Single Molecule Imaging of Genome Duplication and Maintenance Laboratory

Complete and faithful duplication of the genome is crucial to support proper cell division and maintain the genetic integrity of all organisms. In particular, errors in DNA replication can lead to genomic instability, a characteristic feature of most cancer cells.

Our laboratory aims to study eukaryotic DNA replication at the single-molecule level that will lend important new insights into the mechanism and dynamics of the complex molecular machines that carry out genome duplication.

A major advantage of single-molecule methods is that measurements are made on individual molecules, allowing their kinetics to be determined directly. Therefore, one can gain essential information about transient dynamics and heterogeneities which are not accessible using conventional biochemical approaches. In addition, recent advances in nano-manipulation methods allow measuring the physical properties of molecules in response to external forces.

We use a number of model systems including extracts derived from Xenopus leavis eggs and S.cerevisiae as well as the Simian Virus 40 replication system.

A simplified cartoon depiction of the eukaryotic replisome complex.

A simplified cartoon depiction of the eukaryotic replisome complex. (Click to view larger image)

Selected publications

Yardimci H, Walter JC. Prereplication-complex formation: a molecular double take? Nat Struct Mol Biol. 2014; 21:20-25

Yardimci H, Loveland AB., van Oijen AM, Walter JC. Single-molecule analysis of DNA replication in Xenopus egg extracts. Methods, 2012;57:179

Yardimci H, Wang X, Loveland AB, Tappin I, Rudner DZ. Hurwitz J, van Oijen AM, Walter JC.. Bypass of a protein barrier by a replicative DNA helicase. Nature, 2012;492:205

Fu YV, Yardimci H, Long, DT, Ho TV, Guainazzi A, Bermudez, VP, Hurwitz J, van Oijen AM, Scharer O, Walter JC. Selective bypass of a lagging strand roadblock by the eukaryotic replicative DNA helicase. Cell 2011;146:931

Hasan Yardimci

hasan.yardimci@crick.ac.uk
+44 (0) 1707 625821

  • Qualifications and History
  • 2006 PhD, Johns Hopkins University, USA
  • 2008 Postdoctoral fellow, University of Illinois at Urbana-Champaign, USA
  • 2010 Postdoctoral fellow, Harvard Medical School, USA
  • 2013 Establish lab at the London Research Institute, Cancer Research UK
  • 2015 Group Leader, the Francis Crick Institute, London, UK