Martin Singleton

Structural Biology of Chromosome Segregation Laboratory

Our lab is interested in understanding how chromosomes, once replicated, are separated into daughter cells during cell division. It is essential that this process works accurately over many generations, otherwise cells may gain or lose chromosomes, an event that can have severe consequences for the cell, and in turn lead to the onset or progression of diseases such as cancer.

We primarily study these processes by determining the three-dimensional structure of proteins that bind chromosomes using techniques such as X-ray crystallography and electron microscopy. By designing experiments based on the resulting structures, we can gain insights into the way in which the proteins operate in the cell.

Figure 1

Structure of a protein phosphatase subunit bound to the centromere factor, CENP-C (blue). (Click to view larger image)

Selected publications

William C. H. Chao, Yasuto Murayama, Sofía Muñoz, Andrew W. Jones, Benjamin O. Wade, Andrew G. Purkiss, Xiao-Wen Hu, Aaron Borg, Ambrosius P. Snijders, Frank Uhlmann & Martin R. Singleton (2017) Structure of the cohesin loader Scc2 Nature Communications 8, 13952 

Chao, W. C. H., Murayama, Y., Muñoz, S., Costa, A., Uhlmann, F., & Singleton, M. R. (2015). Structural Studies Reveal the Functional Modularity of the Scc2-Scc4 Cohesin Loader.Cell Reports. 12, 719-725

Lipinszki, Z., Lefevre, L., Savoian, M. S., Singleton, M. R., Glover, D. M. and Przewloka, M. R. (2015). Centromeric binding and activity of Protein Phosphatase 4.  Nature Comms. 6, 10.1038/ncomms6894

Drechsler, H., McHugh, T, Singleton, M. R., Carter, N. J. and McAinsh, A. D. (2014). The Kinesin-12 Kif15 is a processive track-switching tetramer. eLife, 3, e01724

Chatterjee A, Zakian S, Hu XW, Singleton MR. Structural insights into cohesion establishment by Wpl1. EMBO J. 2013;32:677-687.

Perriches T, Singleton MR. The structure of the yeast kinetochore Ndc10 DNA-binding domain reveals an unexpected evolutionary relationship to tyrosine recombinases. J Biol Chem. 2012;287(7):5173-5179

Zhang G, Kelstrup CD, Hu XW, Hansen MJ, Singleton MR, Olsen JV, Nilsson J. The Ndc80 internal loop is required for recruitment of the Ska complex to establish end-on microtubule attachment to kinetochores. J Cell Sci. 2012;125,3243-53.

Kingston IJ, Yung JS, Singleton MR. Biophysical Characterization of the Centromere-specific Nucleosome from Budding Yeast. J Biol Chem. 2011;286(5):4021-6

Maskell DP, Hu XW, Singleton MR. Molecular architecture and assembly of the yeast kinetochore MIND complex. J Cell Biol. 2010;190(5):823-34

Purvis A, Singleton MR. Insights into kinetochore-DNA interactions from the structure of Cep3∆. EMBO Reps. 2008;9:56-62

Singleton MR, Dillingham MS, Gaudier M, Kowalczykowski SC, Wigley DB. Crystal structure of RecBCD enzyme reveals a machine for processing DNA breaks. Nature. 2004;432:187-193

Martin Singleton

Martin Singleton

martin.singleton@crick.ac.uk
+44 (0)20 379 62034

  • Qualifications and history
  • 1997 PhD in Biochemistry, University of Exeter, UK
  • 1998 Postdoctoral Research Assistant, Oxford University, UK
  • 2000 Postdoctoral Fellow, Imperial Cancer Research Fund, UK
  • 2005 Established lab at the London Research Institute, Cancer Research UK
  • 2015 Group Leader, the Francis Crick Institute, London, UK