Iris Salecker: Projects

Tools to dissect visual circuit assembly

To facilitate the identification and functional analysis of neuronal and glial subtypes with single cell resolution, we have recently generated three Drosophila variants of the mouse Brainbow-2 system (Livet et al., 2007), called Flybow (FB). This approach enables us to label cells in different colors in the same sample.

It relies on the stochastic expression of membrane-tethered fluorescent proteins by excision and inversion of opposing coding sequences arranged within cassettes. FB combines the Gal4/UAS system and a modified FLP/FRT system with altered specificity to provide (1) precise spatio-temporal control of expression in any genetically accessible cell population and (2) compatibility with available FLP/FRT-system based genetic approaches for functional studies. Our current efforts aim at developing this approach further by adding new sets of FB transgenes to our toolbox.

Figure 1

Figure 1. In the adult visual system, R1-R8 photoreceptor neurons (blue) extend axons from the retina into the lamina and medulla. Neurons in the target area were labeled with the Flybow 2.0 approach.

 

Selected publications

Richier, B. and Salecker, I. (2015) Versatile genetic paintbrushes: Brainbow technologies. WIREs Developmental Biology. 4, 161-180. doi:10.1002/wdev.166.

Shimosako, N., Hadjieconomou, D., and Salecker, I. (2014) Flybow to dissect circuit assembly in the Drosophila brain. Methods Mol. Biol. 1082, 57-69.

Hadjieconomou, D., Rotkopf, S., Alexandre, C., Bell, D.M., Dickson, B.J. and Salecker, I. (2011) Flybow: genetic multicolor cell-labeling for neural circuit analysis in Drosophila melanogaster. Nature Methods 8, 260-266.

Iris Salecker

Iris Salecker

iris.salecker@crick.ac.uk
+44 (0)20 379 62236

  • Qualifications and history
  • 1995 PhD University of Regensburg, Germany
  • 1995 Postdoctoral fellow. University of California Los Angeles, USA
  • 2000 Group Leader, Medical Research Council National Institute for Medical Research, London, UK
  • 2015 Group Leader, the Francis Crick Institute, London, UK