John Diffley: Publications

  • Douglas, ME; Abid Ali, F; Costa, A and Diffley, JFX (2018)
    The mechanism of eukaryotic CMG helicase activation.
    Nature 555, 265-268 PubMed abstract
  • Abid Ali, F; Douglas, ME; Locke, J; Pye, VE; Nans, A; Diffley, JFX and Costa, A (2017)
    Cryo-EM structure of a licensed DNA replication origin.
    Nature Communications 8, 2241 PubMed abstract
  • Coster, G and Diffley, JFX (2017)
    Bidirectional eukaryotic DNA replication is established by quasi-symmetrical helicase loading.
    Science 357, 314-318 PubMed abstract
  • Frigola, J; He, J; Kinkelin, K; Pye, VE; Renault, L; Douglas, ME; Remus, D; Cherepanov, P; Costa, A and Diffley, JFX (2017)
    Cdt1 stabilizes an open MCM ring for helicase loading.
    Nature Communications 8, 15720 PubMed abstract
  • Kurat, CF; Yeeles, JTP; Patel, H; Early, A and Diffley, JFX (2017)
    Chromatin controls DNA replication origin selection, lagging-strand synthesis, and replication fork rates.
    Molecular Cell 65, 117-130 PubMed abstract
  • Yeeles, JTP; Janska, A; Early, A and Diffley, JFX (2017)
    How the eukaryotic replisome achieves rapid and efficient DNA replication.
    Molecular Cell 65, 105-116 PubMed abstract
  • Zhou, JC; Janska, A; Goswami, P; Renault, L; Abid Ali, F; Kotecha, A; Diffley, JFX and Costa, A (2017)
    CMG-Pol epsilon dynamics suggests a mechanism for the establishment of leading-strand synthesis in the eukaryotic replisome.
    Proceedings of the National Academy of Sciences of the United States of America 114, 4141-4146 PubMed abstract
  • Deegan, TD and Diffley, JFX (2016)
    MCM: one ring to rule them all.
    Current Opinion in Structural Biology 37, 145-151 PubMed abstract
  • Deegan, TD; Yeeles, JTP and Diffley, JFX (2016)
    Phosphopeptide binding by Sld3 links Dbf4-dependent kinase to MCM replicative helicase activation.
    EMBO Journal 35, 961-973 PubMed abstract
  • Diffley, JFX (2016)
    On the road to replication.
    EMBO Molecular Medicine 8, 77-79 PubMed abstract
  • Douglas, ME and Diffley, JFX (2016)
    Recruitment of Mcm10 to sites of replication initiation requires direct binding to the minichromosome maintenance (MCM) complex.
    Journal of Biological Chemistry 291, 5879-5888 PubMed abstract
  • Morafraile, EC; Diffley, JFX; Tercero, JA and Segurado, M (2015)
    Checkpoint-dependent RNR induction promotes fork restart after replicative stress.
    Scientific Reports 5, 7886 PubMed abstract
  • Yeeles, JTP; Deegan, TD; Janska, A; Early, A and Diffley, JFX (2015)
    Regulated eukaryotic DNA replication origin firing with purified proteins.
    Nature 519, 431-435 PubMed abstract
  • Coster, G; Frigola, J; Beuron, F; Morris, EP and Diffley, JFX (2014)
    Origin licensing requires ATP binding and hydrolysis by the MCM replicative helicase.
    Molecular Cell 55, 666-677 PubMed abstract
  • Hills, SA and Diffley, JFX (2014)
    DNA replication and oncogene-induced replicative stress.
    Current Biology 24, R435-R444 PubMed abstract
  • On, KF; Beuron, F; Frith, D; Snijders, AP; Morris, EP and Diffley, JFX (2014)
    Prereplicative complexes assembled in vitro support origin-dependent and independent DNA replication.
    EMBO Journal 33, 605-620 PubMed abstract
  • Boos, D; Yekezare, M and Diffley, JFX (2013)
    Identification of a heteromeric complex that promotes DNA replication origin firing in human cells.
    Science 340, 981-984 PubMed abstract
  • Frigola, J; Remus, D; Mehanna, A and Diffley, JFX (2013)
    ATPase-dependent quality control of DNA replication origin licensing.
    Nature 495, 339-343 PubMed abstract
  • Siddiqui, K; On, KF and Diffley, JFX (2013)
    Regulating DNA replication in Eukarya.
    Cold Spring Harbor Perspectives in Biology 5, a012930 PubMed abstract
  • Yekezare, M; Gómez-González, B and Diffley, JFX (2013)
    Controlling DNA replication origins in response to DNA damage - inhibit globally, activate locally.
    Journal of Cell Science 126, 1297-1306 PubMed abstract
  • Boos, D; Frigola, J and Diffley, JFX (2012)
    Activation of the replicative DNA helicase: breaking up is hard to do.
    Current Opinion in Cell Biology 24, 423-430 PubMed abstract
  • Douglas, ME and Diffley, JFX (2012)
    Replication timing: the early bird catches the worm.
    Current Biology 22, R81-R82 PubMed abstract
  • Mehanna, A and Diffley, JFX (2012)
    Pre-replicative complex assembly with purified proteins.
    Methods 57, 222-226 PubMed abstract
  • Baxter, J; Sen, N; Martínez, VL; De Carandini, MEM; Schvartzman, JB; Diffley, JFX and Aragón, L (2011)
    Positive supercoiling of mitotic DNA drives decatenation by topoisomerase II in eukaryotes.
    Science 331, 1328-1332 PubMed abstract
  • Boos, D; Sanchez-Pulido, L; Rappas, M; Pearl, LH; Oliver, AW; Ponting, CP and Diffley, JFX (2011)
    Regulation of DNA replication through Sld3-Dpb11 interaction is conserved from yeast to humans.
    Current Biology 21, 1152-1157 PubMed abstract
  • Diffley, JFX (2011)
    Quality control in the initiation of eukaryotic DNA replication.
    Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences 366, 3545-3553 PubMed abstract
  • Pfander, B and Diffley, JFX (2011)
    Dpb11 coordinates Mec1 kinase activation with cell cycle-regulated Rad9 recruitment.
    EMBO Journal 30, 4897-4907 PubMed abstract
  • Diffley, JFX (2010)
    The many faces of redundancy in DNA replication control.
    Cold Spring Harbor Symposia on Quantitative Biology 75, 135-142 PubMed abstract
  • Sanchez-Pulido, L; Diffley, JFX and Ponting, CP (2010)
    Homology explains the functional similarities of Treslin/Ticrr and Sld3.
    Current Biology 20, R509-R510 PubMed abstract
  • Zegerman, P and Diffley, JFX (2010)
    Checkpoint-dependent inhibition of DNA replication initiation by Sld3 and Dbf4 phosphorylation.
    Nature 467, 474-478 PubMed abstract

[Page last updated 1 Jun 2018]

John Diffley
+44 (0)379 61833

  • Qualifications and history
  • 1985 PhD, New York University, USA
  • 1984 Postdoctoral Fellow, Cold Spring Harbor Laboratory, USA
  • 1990 Established lab at the Imperial Cancer Research Fund, UK (in 2002 the Imperial Cancer Research Fund became Cancer Research UK)
  • 2015 Associate Research Director, the Francis Crick Institute, London, UK