Mike Blackman: Publications

  • Collins, CR; Hackett, F; Atid, J; Tan, MSY and Blackman, MJ (2017)
    The Plasmodium falciparum pseudoprotease SERA5 regulates the kinetics and efficiency of malaria parasite egress from host erythrocytes.
    PLOS Pathogens 13, e1006453 PubMed abstract
  • Diez Benavente, E; Florez de Sessions, P; Moon, RW; Holder, AA; Blackman, MJ; Roper, C; Drakeley, CJ; Pain, A; Sutherland, CJ; Hibberd, ML; Campino, S and Clark, TG (2017)
    Analysis of nuclear and organellar genomes of Plasmodium knowlesi in humans reveals ancient population structure and recent recombination among host-specific subpopulations.
    PLOS Genetics 13, e1007008 PubMed abstract
  • Hale, VL; Watermeyer, JM; Hackett, F; Vizcay-Barrena, G; van Ooij, C; Thomas, JA; Spink, MC; Harkiolaki, M; Duke, E; Fleck, RA; Blackman, MJ and Saibil, HR (2017)
    Parasitophorous vacuole poration precedes its rupture and rapid host erythrocyte cytoskeleton collapse in Plasmodium falciparum egress.
    Proceedings of the National Academy of Sciences of the United States of America 114, 3439-3444 PubMed abstract
  • Knuepfer, E; Napiorkowska, M; van Ooij, C and Holder, AA (2017)
    Generating conditional gene knockouts in Plasmodium - a toolkit to produce stable DiCre recombinase-expressing parasite lines using CRISPR/Cas9.
    Scientific Reports 7, 3881 PubMed abstract
  • Koussis, K; Goulielmaki, E; Chalari, A; Withers-Martinez, C; Siden-Kiamos, I; Matuschewski, K and Loukeris, TG (2017)
    Targeted deletion of a Plasmodium site-2 protease impairs life cycle progression in the mammalian host.
    PLOS ONE 12, e0170260 PubMed abstract
  • Pino, P; Caldelari, R; Mukherjee, B; Vahokoski, J; Klages, N; Maco, B; Collins, CR; Blackman, MJ; Kursula, I; Heussler, V; Brochet, M and Soldati-Favre, D (2017)
    A multistage antimalarial targets the plasmepsins IX and X essential for invasion and egress.
    Science 358, 522-528 PubMed abstract
  • Sherling, ES; Knuepfer, E; Brzostowski, JA; Miller, LH; Blackman, MJ and van Ooij, C (2017)
    The Plasmodium falciparum rhoptry protein RhopH3 plays essential roles in host cell invasion and nutrient uptake.
    eLife 6, 23239 PubMed abstract
  • Brogi, S; Giovani, S; Brindisi, M; Gemma, S; Novellino, E; Campiani, G; Blackman, MJ and Butini, S (2016)
    In silico study of subtilisin-like protease 1 (SUB1) from different Plasmodium species in complex with peptidyl-difluorostatones and characterization of potent pan-SUB1 inhibitors.
    Journal of Molecular Graphics & Modelling 64, 121-130 PubMed abstract
  • Fougère, A; Jackson, AP; Paraskevi Bechtsi, D; Braks, JAM; Annoura, T; Fonager, J; Spaccapelo, R; Ramesar, J; Chevalley-Maurel, S; Klop, O; van der Laan, AMA; Tanke, HJ; Kocken, CHM; Pasini, EM; Khan, SM; Böhme, U; van Ooij, C; Otto, TD; Janse, CJ and Franke-Fayard, B (2016)
    Variant exported blood-stage proteins encoded by Plasmodium multigene families are expressed in liver stages where they are exported into the parasitophorous vacuole.
    PLOS Pathogens 12, e1005917 PubMed abstract
  • Hill, RJ; Ringel, A; Knuepfer, E; Moon, RW; Blackman, MJ and van Ooij, C (2016)
    Regulation and essentiality of the StAR-related lipid transfer (START) domain-containing phospholipid transfer protein PFA0210c in malaria parasites.
    Journal of Biological Chemistry 291, 24280-24292 PubMed abstract
  • Jones, ML; Das, S; Belda, H; Collins, CR; Blackman, MJ and Treeck, M (2016)
    A versatile strategy for rapid conditional genome engineering using loxP sites in a small synthetic intron in Plasmodium falciparum.
    Scientific Reports 6, 21800 PubMed abstract
  • Moon, RW; Sharaf, H; Hastings, CH; Ho, YS; Nair, MB; Rchiad, Z; Knuepfer, E; Ramaprasad, A; Mohring, F; Amir, A; Yusuf, NA; Hall, J; Almond, N; Lau, YL; Pain, A; Blackman, MJ and Holder, AA (2016)
    Normocyte-binding protein required for human erythrocyte invasion by the zoonotic malaria parasite Plasmodium knowlesi.
    Proceedings of the National Academy of Sciences of the United States of America 113, 7231-7236 PubMed abstract
  • Rasina, D; Otikovs, M; Leitans, J; Recacha, R; Borysov, OV; Kanepe-Lapsa, I; Domraceva, I; Pantelejevs, T; Tars, K; Blackman, MJ; Jaudzems, K and Jirgensons, A (2016)
    Fragment-based discovery of 2-aminoquinazolin-4(3H)-ones as novel class nonpeptidomimetic inhibitors of the plasmepsins I, II, and IV.
    Journal of Medicinal Chemistry 59, 374-387 PubMed abstract
  • Sherling, ES and van Ooij, C (2016)
    Host cell remodeling by pathogens: the exomembrane system in Plasmodium-infected erythrocytes.
    FEMS Microbiology Reviews 40, 701-721 PubMed abstract
  • Thomas, JA; Collins, CR; Das, S; Hackett, F; Graindorge, A; Bell, D; Deu, E and Blackman, MJ (2016)
    Development and application of a simple plaque assay for the human malaria parasite Plasmodium falciparum.
    PLOS ONE 11, e0157873 PubMed abstract
  • van Ooij, C (2016)
    Combining traditional medicine and modern chemistry to fight malaria.
    Annals of Translational Medicine 4, 550 PubMed abstract
  • Watermeyer, JM; Hale, VL; Hackett, F; Clare, DK; Cutts, EE; Vakonakis, I; Fleck, RA; Blackman, MJ and Saibil, HR (2016)
    A spiral scaffold underlies cytoadherent knobs in Plasmodium falciparum-infected erythrocytes.
    Blood 127, 343-351 PubMed abstract
  • Das, S; Hertrich, N; Perrin, AJ; Withers-Martinez, C; Collins, CR; Jones, ML; Watermeyer, JM; Fobes, ET; Martin, SR; Saibil, HR; Wright, GJ; Treeck, M; Epp, C and Blackman, MJ (2015)
    Processing of Plasmodium falciparum merozoite surface protein MSP1 activates a spectrin-binding function enabling parasite egress from RBCs.
    Cell Host & Microbe 18, 433-444 PubMed abstract
  • Giovani, S; Penzo, M; Butini, S; Brindisi, M; Gemma, S; Novellino, E; Campiani, G; Blackman, MJ and Brogi, S (2015)
    Plasmodium falciparum subtilisin-like protease 1: discovery of potent difluorostatone-based inhibitors.
    RSC Advances 5, 22431-22448
  • Kher, SS; Penzo, M; Fulle, S; Ebejer, JP; Finn, PW; Blackman, MJ and Jirgensons, A (2015)
    Quinoxaline-based inhibitors of malarial protease PfSUB1.
    Chemistry of Heterocyclic Compounds 50, 1456-1462
  • McConville, M; Fernández, J; Angulo-Barturen, Í; Bahamontes-Rosa, N; Ballell-Pages, L; Castañeda, P; de Cózar, C; Crespo, B; Guijarro, L; Jiménez-Díaz, MB; Martínez-Martínez, MS; de Mercado, J; Santos-Villajero, Á; Sanz, LM; Frigerio, M; Washbourn, G; Ward, SA; Nixon, GL; Biagini, GA; Berry, NG; Blackman, MJ; Calderón, F and O'Neill, PM (2015)
    Carbamoyl triazoles, known serine protease inhibitors, are a potent new class of antimalarials.
    Journal of Medicinal Chemistry 58, 6448-6455 PubMed abstract
  • Pieperhoff, MS; Pall, GS; Jiménez-Ruiz, E; Das, S; Melatti, C; Gow, M; Wong, EH; Heng, J; Müller, S; Blackman, MJ and Meissner, M (2015)
    Conditional U1 gene silencing in Toxoplasma gondii.
    PLOS ONE 10, e0130356 PubMed abstract
  • Stallmach, R; Kavishwar, M; Withers-Martinez, C; Hackett, F; Collins, CR; Howell, SA; Yeoh, S; Knuepfer, E; Atid, AJ; Holder, AA and Blackman, MJ (2015)
    Plasmodium falciparum SERA5 plays a non-enzymatic role in the malarial asexual blood-stage lifecycle.
    Molecular Microbiology 96, 368-387 PubMed abstract
  • Giovani, S; Penzo, M; Brogi, S; Brindisi, M; Gemma, S; Novellino, E; Savini, L; Blackman, MJ; Campiani, G and Butini, S (2014)
    Rational design of the first difluorostatone-based PfSUB1 inhibitors.
    Bioorganic & Medicinal Chemistry Letters 24, 3582-3586 PubMed abstract
  • Grüring, C; Moon, RW; Lim, C; Holder, AA; Blackman, MJ and Duraisingh, MT (2014)
    Human red blood cell-adapted Plasmodium knowlesi parasites: a new model system for malaria research.
    Cellular Microbiology 16, 612-620 PubMed abstract
  • Hackett, F; Collins, CR; Strath, M and Blackman, MJ (2014)
    Cyclic nucleotide (cAMP and cGMP) assays and capture ELISA for quantitative analysis of Plasmodium falciparum blood-stage egress.
    Bio-Protocol 4, e1055 PubMed abstract
  • Jaudzems, K; Tars, K; Maurops, G; Ivdra, N; Otikovs, M; Leitans, J; Kanepe-Lapsa, I; Domraceva, I; Mutule, I; Trapencieris, P; Blackman, MJ and Jirgensons, A (2014)
    Plasmepsin inhibitory activity and structure-guided optimization of a potent hydroxyethylamine-based antimalarial hit.
    ACS Medicinal Chemistry Letters 5, 373-377 PubMed abstract
  • Kher, SS; Penzo, M; Fulle, S; Finn, PW; Blackman, MJ and Jirgensons, A (2014)
    Substrate derived peptidic α-ketoamides as inhibitors of the malarial protease PfSUB1.
    Bioorganic & Medicinal Chemistry Letters 24, 4486-4489 PubMed abstract
  • Withers-Martinez, C; Strath, M; Hackett, F; Haire, LF; Howell, SA; Walker, PA; Christodoulou, E; Dodson, GG and Blackman, MJ (2014)
    The malaria parasite egress protease SUB1 is a calcium-dependent redox switch subtilisin.
    Nature Communications 5, 3726 PubMed abstract
  • Blackman, MJ and Carruthers, VB (2013)
    Recent insights into apicomplexan parasite egress provide new views to a kill.
    Current Opinion in Microbiology 16, 459-464 PubMed abstract
  • Child, MA; Harris, PK; Collins, CR; Withers-Martinez, C; Yeoh, S and Blackman, MJ (2013)
    Molecular determinants for subcellular trafficking of the malarial sheddase PfSUB2.
    Traffic 14, 1053-1064 PubMed abstract
  • Collins, CR; Das, S; Wong, EH; Andenmatten, N; Stallmach, R; Hackett, F; Herman, J-P; Müller, S; Meissner, M and Blackman, MJ (2013)
    Robust inducible Cre recombinase activity in the human malaria parasite Plasmodium falciparum enables efficient gene deletion within a single asexual erythrocytic growth cycle.
    Molecular Microbiology 88, 687-701 PubMed abstract
  • Collins, CR; Hackett, F; Strath, M; Penzo, M; Withers-Martinez, C; Baker, DA and Blackman, MJ (2013)
    Malaria parasite cGMP-dependent protein kinase regulates blood stage merozoite secretory organelle discharge and egress.
    PLOS Pathogens 9, e1003344 PubMed abstract
  • Deligianni, E; Morgan, RN; Bertuccini, L; Wirth, CC; Silmon de Monerri, NC; Spanos, L; Blackman, MJ; Louis, C; Pradel, G and Siden-Kiamos, I (2013)
    A perforin-like protein mediates disruption of the erythrocyte membrane during egress of Plasmodium berghei male gametocytes.
    Cellular Microbiology 15, 1438-1455 PubMed abstract
  • Fulle, S; Withers-Martinez, C; Blackman, MJ; Morris, GM and Finn, PW (2013)
    Molecular determinants of binding to the Plasmodium subtilisin-like protease 1.
    Journal of Chemical Information and Modeling 53, 573-583 PubMed abstract
  • Moon, RW; Hall, J; Rangkuti, F; Ho, YS; Almond, N; Mitchell, GH; Pain, A; Holder, AA and Blackman, MJ (2013)
    Adaptation of the genetically tractable malaria pathogen Plasmodium knowlesi to continuous culture in human erythrocytes.
    Proceedings of the National Academy of Sciences of the United States of America 110, 531-536 PubMed abstract
  • Suarez, C; Volkmann, K; Gomes, AR; Billker, O and Blackman, MJ (2013)
    The malarial serine protease SUB1 plays an essential role in parasite liver stage development.
    PLOS Pathogens 9, e1003811 PubMed abstract
  • van Ooij, C; Withers-Martinez, C; Ringel, A; Cockcroft, S; Haldar, K and Blackman, MJ (2013)
    Identification of a Plasmodium falciparum phospholipid transfer protein.
    Journal of Biological Chemistry 288, 31971-31983 PubMed abstract
  • Collins, CR and Blackman, MJ (2012)
    Apicomplexan AMA1 in host cell invasion: a model at the junction?
    Cell Host & Microbe 10, 531-533 PubMed abstract
  • da Cruz, LN; Juliano, MA; Budu, A; Juliano, L; Holder, AA; Blackman, MJ and Garcia, CRS (2012)
    Extracellular ATP triggers proteolysis and cytosolic Ca2+ rise in Plasmodium berghei and Plasmodium yoelii malaria parasites.
    Malaria Journal 11, 69 PubMed abstract
  • Gemma, S; Giovani, S; Brindisi, M; Tripaldi, P; Brogi, S; Savini, L; Fiorini, I; Novellino, E; Butini, S; Campiani, G; Penzo, M and Blackman, MJ (2012)
    Quinolylhydrazones as novel inhibitors of Plasmodium falciparum serine protease PfSUB1.
    Bioorganic & Medicinal Chemistry Letters 22, 5317-5321 PubMed abstract
  • Ruecker, A; Shea, M; Hackett, F; Suarez, C; Hirst, EMA; Milutinovic, K; Withers-Martinez, C and Blackman, MJ (2012)
    Proteolytic activation of the essential parasitophorous vacuole cysteine protease SERA6 accompanies malaria parasite egress from its host erythrocyte.
    Journal of Biological Chemistry 287, 37949-37963 PubMed abstract
  • Withers-Martinez, C; Suarez, C; Fulle, S; Kher, S; Penzo, M; Ebejer, J-P; Koussis, K; Hackett, F; Jirgensons, A; Finn, P and Blackman, MJ (2012)
    Plasmodium subtilisin-like protease 1 (SUB1): insights into the active-site structure, specificity and function of a pan-malaria drug target.
    International Journal for Parasitology 42, 597-612 PubMed abstract
  • Olivieri, A; Collins, CR; Hackett, F; Withers-Martinez, C; Marshall, J; Flynn, HR; Skehel, JM and Blackman, MJ (2011)
    Juxtamembrane shedding of Plasmodium falciparum AMA1 is sequence independent and essential, and helps evade invasion-inhibitory antibodies.
    PLOS Pathogens 7, e1002448 PubMed abstract
  • Santos, JM; Ferguson, DJP; Blackman, MJ and Soldati-Favre, D (2011)
    Intramembrane cleavage of AMA1 triggers Toxoplasma to switch from an invasive to a replicative mode.
    Science 331, 473-477 PubMed abstract
  • Silmon de Monerri, NC; Flynn, HR; Campos, MG; Hackett, F; Koussis, K; Withers-Martinez, C; Skehel, JM and Blackman, MJ (2011)
    Global identification of multiple substrates for Plasmodium falciparum SUB1, an essential malarial processing protease.
    Infection and Immunity 79, 1086-1097 PubMed abstract
  • Child, MA; Epp, C; Bujard, H and Blackman, MJ (2010)
    Regulated maturation of malaria merozoite surface protein-1 is essential for parasite growth.
    Molecular Microbiology 78, 187-202 PubMed abstract
  • Dvorin, JD; Martyn, DC; Patel, SD; Grimley, JS; Collins, CR; Hopp, CS; Bright, AT; Westenberger, S; Winzeler, E; Blackman, MJ; Baker, DA; Wandless, TJ and Duraisingh, MT (2010)
    A plant-like kinase in Plasmodium falciparum regulates parasite egress from erythrocytes.
    Science 328, 910-912 PubMed abstract
  • Friedrich, N; Santos, JM; Liu, Y; Palma, AS; Leon, E; Saouros, S; Kiso, M; Blackman, MJ; Matthews, S; Feizi, T and Soldati-Favre, D (2010)
    Members of a novel protein family containing microneme adhesive repeat domains act as sialic acid-binding lectins during host cell invasion by Apicomplexan parasites.
    Journal of Biological Chemistry 285, 2064-2076 PubMed abstract
  • Woehlbier, U; Epp, C; Hackett, F; Blackman, MJ and Bujard, H (2010)
    Antibodies against multiple merozoite surface antigens of the human malaria parasite Plasmodium falciparum inhibit parasite maturation and red blood cell invasion.
    Malaria Journal 9, 77 PubMed abstract

[Page last updated 1 Nov 2017]

Mike Blackman

Mike Blackman

mike.blackman@crick.ac.uk
+44 (0)20 379 62328

  • Qualifications and history
  • 1981 BSc Microbiology, University of Leeds, UK
  • 1985 MSc Immunology, University of Warwick, UK
  • 1985 RA MRC Laboratories, The Gambia.
  • 1991 PhD MRC-NIMR, London, UK
  • 2000 Group Leader, Medical Research Council National Institute for Medical Research, London, UK
  • 2015 Group Leader, the Francis Crick Institute, London, UK