Scientists have discovered how the production of different types
of nerve cell and the growth of the spinal cord are coordinated
during the development of embryos.
The research was led by scientists at the Medical Research
Council's National Institute for Medical Research (NIMR; now
part of the Francis Crick Institute).
Embryonic development requires the production of new cells with
specific identities and functions. But until now, scientists didn't
know how a growing tissue establishes and maintains the right
proportions of different cell types.
Dr James Briscoe's team at NIMR addressed this question by
studying the development of nerve cells, or neurons, in mouse and
chicken embryos.
Progenitor cells are cells that can generate one or more cell
types but cannot divide and reproduce indefinitely. In this work,
the scientists showed that the proportions of progenitor cells that
give rise to different types of spinal cord neurons change during
embryonic development.
These changes in proportions were similar in both mouse and
chick embryos, and in a mutant strain of mouse that is smaller than
normal.
The changes in progenitor cell proportions couldn't be explained
by differences in the rate of cell division. Instead, the
researchers revealed that different mechanisms are employed during
two distinct phases of spinal cord development. Initially,
morphogen gradients - long range signals that partition the tissue
into domains of distinct cell types - establish the pattern and
proportion of different cell types.
During the second phase, the growth of these domains is
controlled by the speed at which progenitor cells differentiate to
become neurons. It is this regulation of differentiation rate that
appears to account for the proportions of different domains and
accommodate variations in size.
Anna Kicheva of NIMR said: "The use of quantitative methods has
allowed us to measure, for the first time, how the number of each
type of neural progenitor cell in the spinal cord changes during
embryonic development. Our data reveal that the proportions of
different cell types are controlled using different mechanisms at
different developmental times and that the rate of differentiation
is key to explaining the final pattern."
Dr Briscoe added: "Our study provides new insight into the
long-standing question of how growth is coordinated with the
production of different types of cells in developing tissues. It's
likely that similar strategies are used in other developing
tissues.
"Our findings might help to inform tissue engineering research -
which aims to artificially grow tissues to repair or replace
diseased or damaged tissues in patients."
The paper, Coordination of progenitor specification and growth in mouse and
chick spinal cord, is published inScience.